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Histopatholgic and immunohistochemical analysis of tumor specimens from GL261 and GL261.luc bearing animals. Hematoxylin and Eosin (H&E) staining was used for conventional morphologic analysis of tumor. Ki-67 staining was for examining tumor cell proliferation, whereas C3 and CD31 staining was for T-cell infiltration and tumor neovascularization, respectively. Magnification is 200 X.

Journal: Journal of Translational Medicine

Article Title: Stable luciferase expression does not alter immunologic or in vivo growth properties of GL261 murine glioma cells

doi: 10.1186/s12967-014-0345-4

Figure Lengend Snippet: Histopatholgic and immunohistochemical analysis of tumor specimens from GL261 and GL261.luc bearing animals. Hematoxylin and Eosin (H&E) staining was used for conventional morphologic analysis of tumor. Ki-67 staining was for examining tumor cell proliferation, whereas C3 and CD31 staining was for T-cell infiltration and tumor neovascularization, respectively. Magnification is 200 X.

Article Snippet: The following antibodies are used for detection of (i) Ki-67 [monoclonal rat anti-mouse Ki-67 antigen, clone TEC-3 (1:31250) (DAKO M7249) DAKO, Glostrup, Denmark] (ii) CD31 [goat polyclonal anti-mouse CD31 antigen, clone M-20 (1:1000) (sc-1506) Santa Cruz Biotechnology, Dallas, Texas U.S.A] and (iii) CD3 [Rabbit Polyclonal anti-mouse CD3 antigen, clone F7.2.38 (1:200) (Dako A0452) DAKO, Glostrup, Denmark] using DAKO auto-stainer.

Techniques: Immunohistochemical staining, Staining

Luciferase expression does not significantly impact histopathologic characterization of GL261 cells

Journal: Journal of Translational Medicine

Article Title: Stable luciferase expression does not alter immunologic or in vivo growth properties of GL261 murine glioma cells

doi: 10.1186/s12967-014-0345-4

Figure Lengend Snippet: Luciferase expression does not significantly impact histopathologic characterization of GL261 cells

Article Snippet: The following antibodies are used for detection of (i) Ki-67 [monoclonal rat anti-mouse Ki-67 antigen, clone TEC-3 (1:31250) (DAKO M7249) DAKO, Glostrup, Denmark] (ii) CD31 [goat polyclonal anti-mouse CD31 antigen, clone M-20 (1:1000) (sc-1506) Santa Cruz Biotechnology, Dallas, Texas U.S.A] and (iii) CD3 [Rabbit Polyclonal anti-mouse CD3 antigen, clone F7.2.38 (1:200) (Dako A0452) DAKO, Glostrup, Denmark] using DAKO auto-stainer.

Techniques: Luciferase, Expressing